Hotspot

psbB-psbT

LSC · 81805-82550 · 746 bp

Back to Rhizophora

Pi 0.0204

MarkerSeek score 50.80

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 11
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.48
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GTGCTCGAACCTTGTTCAGA	CTTTAGGCGGTTCTCGGAAA	50.0	50.0	58.1	57.9	563-950 bp	1.0	83.5
psbB-psbT_p2	GTGCTCGAACCTTGTTCAGA	TAGGCGGTTCTCGGAAAAAG	50.0	50.0	58.1	57.9	560-947 bp	1.0	83.5
psbB-psbT_p3	TGCTCGAACCTTGTTCAGAG	CTTTAGGCGGTTCTCGGAAA	50.0	50.0	57.8	57.9	562-949 bp	1.0	83.3
psbB-psbT_p4	TGCTCGAACCTTGTTCAGAG	TAGGCGGTTCTCGGAAAAAG	50.0	50.0	57.8	57.9	559-946 bp	1.0	83.3
psbB-psbT_p5	GCTCGAACCTTGTTCAGAGA	TAGGCGGTTCTCGGAAAAAG	50.0	50.0	57.6	57.9	558-945 bp	1.0	81.0