Hotspot

psbE-petL

LSC · 71551-72952 · 1402 bp

Back to Rhizophora

Pi 0.00143

MarkerSeek score 60.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1402
variable_sites: 3
pi: 0.00143
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1517-1538 bp	1.0	71.6
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1512-1533 bp	1.0	71.3
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.4	57.1	1510-1531 bp	1.0	70.1
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCAA	50.0	47.6	57.8	57.8	1517-1538 bp	1.0	69.3
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	1518-1539 bp	1.0	69.3