Hotspot

psaA-ycf3

LSC · 47446-48274 · 829 bp

Back to Rhizophora

Pi 0.00493

MarkerSeek score 63.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 6
pi: 0.00493
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTCGATCAAGCCGCTGAATA	50.0	45.0	57.9	57.4	1011-1017 bp	1.0	76.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TGAAGCCCAGAATTGGTTGA	50.0	45.0	57.9	57.3	952-958 bp	1.0	75.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TCAAGCCGCTGAATATTGGA	50.0	45.0	57.9	57.3	1006-1012 bp	1.0	75.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TCGATCAAGCCGCTGAATAT	50.0	45.0	57.9	57.2	1010-1016 bp	1.0	74.3
psaA-ycf3_p5	TGTTCTTGAGAAATGCCCCG	TTCGATCAAGCCGCTGAATA	50.0	45.0	58.5	57.4	1036-1042 bp	1.0	73.3