Hotspot

atpA-atpF

LSC · 12715-12769 · 55 bp

Back to Rhizophora

Pi 0.0128

MarkerSeek score 64.80

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 55
variable_sites: 1
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	AATCTCATCGGCTCGAATGG	TGGTATGTTTGGGGCAATGA	50.0	45.0	57.8	57.4	115-119 bp	1.0	77.2
atpA-atpF_p2	TCATCGGCTCGAATGGTTAC	TGGTATGTTTGGGGCAATGA	50.0	45.0	57.8	57.4	111-115 bp	1.0	76.9
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	TGGTATGTTTGGGGCAATGA	55.0	45.0	58.1	57.4	201-205 bp	1.0	76.3
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	TGGTATGTTTGGGGCAATGA	55.0	45.0	58.1	57.4	199-203 bp	1.0	76.3
atpA-atpF_p5	TCGCCTACTTGAAGTACGGT	TGGTATGTTTGGGGCAATGA	50.0	45.0	58.5	57.4	198-202 bp	1.0	73.4