Hotspot

trnK-UUU-trnQ-UUG

LSC · 5201-6702 · 1502 bp

Back to Rhizophora

Pi 0.0128

MarkerSeek score 70.80

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1502
variable_sites: 27
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1573-2104 bp	1.0	83.2
trnK-UUU-trnQ-UUG_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1572-2103 bp	1.0	83.2
trnK-UUU-trnQ-UUG_p3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1559-2090 bp	1.0	80.8
trnK-UUU-trnQ-UUG_p4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	1584-2115 bp	1.0	79.9
trnK-UUU-trnQ-UUG_p5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	1582-2113 bp	1.0	78.7