Hotspot

ycf1

Genome · 88772-94501 · 5730 bp

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Pi 0.0756

MarkerSeek score 73.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5730
variable_sites: 420
pi: 0.0756
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCGTATTGGAGCCTGGATC	ACAGAACACCTAGGTCGACA	55.0	50.0	57.8	58.0	642 bp	0.5	58.7
ycf1_p2	GTCGTATTGGAGCCTGGATC	TCCTCCTTCTCCACCCAATT	55.0	50.0	57.8	57.9	610 bp	0.5	58.3
ycf1_p3	GTCGTATTGGAGCCTGGATC	CGCCCTTTTTCTTGTTGCAA	55.0	45.0	57.8	58.1	670 bp	0.5	58.2
ycf1_p4	GTCGTATTGGAGCCTGGATC	ACCCTTTTCGCCCTTTTTCT	55.0	45.0	57.8	57.9	678 bp	0.5	58.1
ycf1_p5	GTCGTATTGGAGCCTGGATC	CTCCGTTTTGCAGCAATGTT	55.0	45.0	57.8	57.9	771 bp	0.5	57.6