Hotspot

trnI-CAU-trnH-GUG

Genome · 121860-122055 · 196 bp

Back to Rhipsalis

Pi 0.0745
MarkerSeek score 77.00
Primer available yes
Label trnI-CAU-trnH-GUG

Feature Metadata

label_name
trnI-CAU-trnH-GUG
feature_type
igs
region
Genome
length_bp
196
variable_sites
12
pi
0.0745
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-trnH-GUG_p1 GAATGGTTAAAGCGCCCAAC CGGATGTAGCCAAGTGGATC 50.0 55.0 58.0 58.1 331 bp 0.5 58.9
trnI-CAU-trnH-GUG_p2 GAATGGTTAAAGCGCCCAAC TGGATCAAGGCAGTGGATTG 50.0 50.0 58.0 57.9 317 bp 0.5 58.9
trnI-CAU-trnH-GUG_p3 GAATGGTTAAAGCGCCCAAC GGATCAAGGCAGTGGATTGT 50.0 50.0 58.0 57.9 316 bp 0.5 58.8
trnI-CAU-trnH-GUG_p4 GAATGGTTAAAGCGCCCAAC GTGGATCAAGGCAGTGGATT 50.0 50.0 58.0 57.9 318 bp 0.5 58.8
trnI-CAU-trnH-GUG_p5 GCTAAGCATCCATGGCTGAA CGGATGTAGCCAAGTGGATC 50.0 55.0 58.3 58.1 348 bp 0.5 56.4