Hotspot

rps7-trnL-CAA

Genome · 112671-115153 · 2483 bp

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Pi 0.125

MarkerSeek score 81.80

Primer available yes

Label rps7-trnL-CAA

Feature Metadata

label_name: rps7-trnL-CAA
feature_type: igs
region: Genome
length_bp: 2483
variable_sites: 265
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-trnL-CAA_p1	CTTTGGCACATTTTCGTCGT	GTCTCGCGTGTCTACCATTT	45.0	50.0	57.6	58.0	2407-2538 bp	1.0	88.7
rps7-trnL-CAA_p2	CTTTGGCACATTTTCGTCGT	CTCGCGTGTCTACCATTTCA	45.0	50.0	57.6	58.0	2405-2536 bp	1.0	88.7
rps7-trnL-CAA_p3	AGCTTTGGCACATTTTCGTC	GTCTCGCGTGTCTACCATTT	45.0	50.0	57.6	58.0	2409-2540 bp	1.0	88.6
rps7-trnL-CAA_p4	GAGCTTTGGCACATTTTCGT	GTCTCGCGTGTCTACCATTT	45.0	50.0	57.6	58.0	2410-2541 bp	1.0	88.6
rps7-trnL-CAA_p5	AGCTTTGGCACATTTTCGTC	CTCGCGTGTCTACCATTTCA	45.0	50.0	57.6	58.0	2407-2538 bp	1.0	88.5