Hotspot

petN-psbM

Genome · 26615-27290 · 676 bp

Back to Rhipsalis

Pi 0.17

MarkerSeek score 80.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 676
variable_sites: 90
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTTAGCCAAAATGA	50.0	35.0	58.3	54.0	656-784 bp	1.0	57.8
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAACGGTTAGCCAAAATGA	50.0	35.0	57.6	54.0	594-722 bp	1.0	57.0
petN-psbM_p3	ATTGTAAGTCTCGCTTGGGC	AAACGGTTAGCCAAAATGAT	50.0	35.0	58.3	53.7	655-783 bp	1.0	55.8
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	AACGGTTAGCCAAAATGATT	50.0	35.0	58.3	53.7	654-782 bp	1.0	55.8
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AACGGTTAGCCAAAATGATT	50.0	35.0	57.6	53.7	592-720 bp	1.0	55.1