Hotspot

rpoC1-rpoB

Genome · 21825-21853 · 29 bp

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Pi 0.103
MarkerSeek score 74.80
Primer available yes
Label rpoC1-rpoB

Feature Metadata

label_name
rpoC1-rpoB
feature_type
igs
region
Genome
length_bp
29
variable_sites
3
pi
0.103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-rpoB_p1 TGGCCCAAGCACTTATTTGT TCCAGATTAATAGGATGGAAGCT 45.0 39.1 58.0 56.6 128 bp 1.0 46.3
rpoC1-rpoB_p2 TGTGGCCCAAGCACTTATTT TCCAGATTAATAGGATGGAAGCT 45.0 39.1 58.0 56.6 130 bp 1.0 46.3
rpoC1-rpoB_p3 TTGTGGCCCAAGCACTTATT TCCAGATTAATAGGATGGAAGCT 45.0 39.1 58.0 56.6 131 bp 1.0 46.2
rpoC1-rpoB_p4 TTTGTGGCCCAAGCACTTAT TCCAGATTAATAGGATGGAAGCT 45.0 39.1 58.0 56.6 132 bp 1.0 46.2
rpoC1-rpoB_p5 TTTTGTGGCCCAAGCACTTA TCCAGATTAATAGGATGGAAGCT 45.0 39.1 58.2 56.6 133 bp 1.0 44.5