Hotspot

trnS-GCU-clpP

Genome · 7219-7642 · 424 bp

Back to Rhipsalis

Pi 0.0597
MarkerSeek score 73.70
Primer available yes
Label trnS-GCU-clpP

Feature Metadata

label_name
trnS-GCU-clpP
feature_type
igs
region
Genome
length_bp
424
variable_sites
23
pi
0.0597
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-clpP_p1 ACGGAAAGAGAGGGATTCGA TATCCTAGCGTGAGGGAGTG 50.0 55.0 58.2 58.0 853-891 bp 1.0 88.7
trnS-GCU-clpP_p2 ACGGAAAGAGAGGGATTCGA ATTCTCCTCCGACCAAGACA 50.0 50.0 58.2 58.1 818-856 bp 1.0 88.5
trnS-GCU-clpP_p3 ACGGAAAGAGAGGGATTCGA TTCTCCTCCGACCAAGACAT 50.0 50.0 58.2 58.1 817-855 bp 1.0 88.5
trnS-GCU-clpP_p4 ACGGAAAGAGAGGGATTCGA CTTGTTAGATCACGCCACGA 50.0 50.0 58.2 58.0 596 bp 0.5 58.7
trnS-GCU-clpP_p5 ACGGAAAGAGAGGGATTCGA TTGTTAGATCACGCCACGAG 50.0 50.0 58.2 58.0 595 bp 0.5 58.7