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rps16-trnQ-UUG

Genome · 5471-5730 · 260 bp

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Pi 0.0545

MarkerSeek score 81.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 260
variable_sites: 14
pi: 0.0545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	333-485 bp	1.0	81.6
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	344-496 bp	1.0	81.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	363-515 bp	1.0	80.6
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.2	58.2	374-526 bp	1.0	80.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTAAGGCATCGGGTTTTGGT	45.0	50.0	58.1	58.5	364-516 bp	1.0	78.3