Hotspot

ycf1

IRb · 107062-108726 · 1665 bp

Back to Rhinanthus

Pi 0.0032

MarkerSeek score 60.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1665
variable_sites: 8
pi: 0.0032
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGATGTCGTTTCTAGTCT	TTTCAGTACTCCCATGTTGG	40.0	45.0	53.5	55.0	1807-1839 bp	1.0	40.4
ycf1_p2	ACAGATGTCGTTTCTAGTCT	TTTTCAGTACTCCCATGTTGG	40.0	42.9	53.5	55.8	1808-1840 bp	1.0	40.4
ycf1_p3	ACAGATGTCGTTTCTAGTCT	TTTTTCAGTACTCCCATGTTGG	40.0	40.9	53.5	56.5	1809-1841 bp	1.0	40.4
ycf1_p4	AGAAATAAGGGAGGTTTGTG	TTTCAGTACTCCCATGTTGG	40.0	45.0	52.7	55.0	1707-1739 bp	1.0	40.4
ycf1_p5	ACAGATGTCGTTTCTAGTCTATCT	TTTCAGTACTCCCATGTTGG	37.5	45.0	56.7	55.0	1807-1839 bp	1.0	40.4