Hotspot

psbB

LSC · 71750-73276 · 1527 bp

Back to Rhinanthus

Pi 0.00131

MarkerSeek score 59.50

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	CCGAAGAAGGAGAGTCCAGA	GCGAATGATACCAAAGCAGC	55.0	50.0	58.2	57.9	1645-1655 bp	1.0	78.5
psbB_p2	TCCGAAGAAGGAGAGTCCAG	GCGAATGATACCAAAGCAGC	55.0	50.0	58.2	57.9	1646-1656 bp	1.0	78.5
psbB_p3	CCGAAGAAGGAGAGTCCAGA	AATGATACCAAAGCAGCGGT	55.0	45.0	58.2	57.9	1642-1652 bp	1.0	78.3
psbB_p4	TCCGAAGAAGGAGAGTCCAG	AATGATACCAAAGCAGCGGT	55.0	45.0	58.2	57.9	1643-1653 bp	1.0	78.3
psbB_p5	TTCCGAAGAAGGAGAGTCCA	GCGAATGATACCAAAGCAGC	50.0	50.0	57.7	57.9	1647-1657 bp	1.0	77.3