Hotspot

clpP1

LSC · 69385-71330 · 1946 bp

Back to Rhinanthus

Pi 0.00171

MarkerSeek score 45.80

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 1946
variable_sites: 5
pi: 0.00171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	ACGGGTTTCAACAAAATATATT	ACGTTTCCACATCAAAGTGA	27.3	40.0	52.3	55.5	2020-2021 bp	1.0	40.2
clpP1_p2	ACGGGTTTCAACAAAATATATTT	ACGTTTCCACATCAAAGTGA	26.1	40.0	53.2	55.5	2020-2021 bp	1.0	40.2
clpP1_p3	ACGGGTTTCAACAAAATATATT	ACGTTTCCACATCAAAGTGAA	27.3	38.1	52.3	56.3	2020-2021 bp	1.0	40.2
clpP1_p4	ACGGGTTTCAACAAAATATATTTT	ACGTTTCCACATCAAAGTGA	25.0	40.0	54.0	55.5	2020-2021 bp	1.0	40.2
clpP1_p5	ACGGGTTTCAACAAAATATATTT	ACGTTTCCACATCAAAGTGAA	26.1	38.1	53.2	56.3	2020-2021 bp	1.0	40.2