Hotspot

rpl32-trnL-UAG

SSC · 109759-110519 · 761 bp

Back to Rhinanthus

Pi 0.00263

MarkerSeek score 60.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 761
variable_sites: 3
pi: 0.00263
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGTAGGGGGTATTGGACAG	CTCGCACTGCTTCCTAAGAG	55.0	55.0	57.8	58.1	911 bp	1.0	78.1
rpl32-trnL-UAG_p2	AGGTAGGGGGTATTGGACAG	CTCTCGCACTGCTTCCTAAG	55.0	55.0	57.8	58.1	913 bp	1.0	78.1
rpl32-trnL-UAG_p3	AGGTAGGGGGTATTGGACAG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	57.8	57.9	900 bp	1.0	77.7
rpl32-trnL-UAG_p4	AGGTAGGGGGTATTGGACAG	AGCAGCGTGTCTACCAATTT	55.0	45.0	57.8	57.8	893 bp	1.0	77.3
rpl32-trnL-UAG_p5	AGGTAGGGGGTATTGGACAG	CTAAGAGCAGCGTGTCTACC	55.0	55.0	57.8	57.8	898 bp	1.0	77.2