Hotspot

psbT-psbN

LSC · 73568-73628 · 61 bp

Back to Rhinanthus

Pi 0.0328

MarkerSeek score 60.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 3
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	127 bp	1.0	67.3
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	AGATCCATTCGAGGAACACG	47.6	50.0	57.6	57.4	128 bp	1.0	66.0
psbT-psbN_p3	TCGAGAACCACCTAAAGTTCC	GATCCATTCGAGGAACACGG	47.6	55.0	57.3	58.4	128 bp	1.0	65.1
psbT-psbN_p4	TCGAGAACCACCTAAAGTTCC	AGATCCATTCGAGGAACACG	47.6	50.0	57.3	57.4	129 bp	1.0	63.8
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	CGAGATCCATTCGAGGAACA	47.6	50.0	57.6	57.1	130 bp	1.0	63.7