Hotspot

accD-psaI

LSC · 58442-59072 · 631 bp

Back to Rhinanthus

Pi 0.00211

MarkerSeek score 60.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 2
pi: 0.00211
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCGTACCGCGCAATCTTTTA	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	790 bp	1.0	79.9
accD-psaI_p2	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	813 bp	1.0	79.6
accD-psaI_p3	TCGTACCGCGCAATCTTTTA	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	796 bp	1.0	79.3
accD-psaI_p4	CCAGAAGGGCTTATTCGACC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	819 bp	1.0	79.0
accD-psaI_p5	TAATCGTACCGCGCAATCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.8	58.0	793 bp	1.0	78.3