Hotspot

atpB-rbcL

LSC · 54204-54963 · 760 bp

Back to Rhinanthus

Pi 0.00263

MarkerSeek score 47.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 3
pi: 0.00263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GACCGATGATTTGGACCACA	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	896 bp	1.0	77.0
atpB-rbcL_p2	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	921 bp	1.0	76.6
atpB-rbcL_p3	ACCGATGATTTGGACCACAC	AACACCCGCTTTGAATCCAA	50.0	45.0	58.2	58.3	895 bp	1.0	76.4
atpB-rbcL_p4	TGGAAACCCCAGGATCAGAA	AACACCCGCTTTGAATCCAA	50.0	45.0	58.2	58.3	851 bp	1.0	75.9
atpB-rbcL_p5	CATCTTACCTGGCGGAAAGG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.3	58.3	931 bp	1.0	75.2