Hotspot

ndhC-trnV-UAC

LSC · 49965-51182 · 1218 bp

Back to Rhinanthus

Pi 0.00109

MarkerSeek score 60.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 2
pi: 0.00109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AAATTGTAACCAAGCGTCGC	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.7	57.8	1267-1275 bp	1.0	76.4
ndhC-trnV-UAC_p2	AAATTGTAACCAAGCGTCGC	GAAGGTCTACGGTTCGAGTC	45.0	55.0	57.7	57.5	1266-1274 bp	1.0	73.7
ndhC-trnV-UAC_p3	AAATTGTAACCAAGCGTCGC	GTTCGAGTCCGTATAGCCCT	45.0	55.0	57.7	58.7	1255-1263 bp	1.0	72.6
ndhC-trnV-UAC_p4	AAATTGTAACCAAGCGTCGC	AAGGTCTACGGTTCGAGTCC	45.0	55.0	57.7	58.8	1265-1273 bp	1.0	71.4
ndhC-trnV-UAC_p5	AAATTGTAACCAAGCGTCGC	GAGAAGGTCTACGGTTCGAG	45.0	55.0	57.7	57.2	1268-1276 bp	1.0	71.4