Hotspot

ndhG

SSC · 125348-125878 · 531 bp

Back to Rheum

Pi 0.00661
MarkerSeek score 40.10
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
17
pi
0.00661
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 ACAATAGCCGGTCCAATAGC TGCGGTAATTGCGTTGAGTA 50.0 45.0 57.7 57.9 1516-1556 bp 1.0 84.2
ndhG_p2 ACAATAGCCGGTCCAATAGC TGCTTTAGGCCGTTTACCAA 50.0 45.0 57.7 57.7 1415-1455 bp 1.0 83.1
ndhG_p3 ACAATAGCCGGTCCAATAGC TTGCTTTAGGCCGTTTACCA 50.0 45.0 57.7 57.7 1416-1456 bp 1.0 83.1
ndhG_p4 GCAAAGACCAGACGAATCGA TGCGGTAATTGCGTTGAGTA 50.0 45.0 58.3 57.9 1748-1801 bp 0.947368 82.9
ndhG_p5 AAGTTTGATTTGGCGTGACG TGCGGTAATTGCGTTGAGTA 45.0 45.0 57.6 57.9 1112-1117 bp 0.789474 70.9