Hotspot

clpP

LSC · 71781-74176 · 2396 bp

Back to Rheum

Pi 0.016
MarkerSeek score 59.40
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2396
variable_sites
142
pi
0.016
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 GTGACGTTTCGAATTGGCTG GCAAATGCCGCCATATTTGT 50.0 45.0 58.0 58.1 2697-2849 bp 1.0 87.7
clpP_p2 GTGACGTTTCGAATTGGCTG CTCGGCGCAAGGGTTAATAT 50.0 50.0 58.0 58.1 2837-2988 bp 0.842105 80.3
clpP_p3 GTGACGTTTCGAATTGGCTG GCCGCCATATTTGTTCATCG 50.0 50.0 58.0 58.0 2790 bp 0.052632 41.4
clpP_p4 ACGTTTCGAATTGGCTGTCT GCCGCCATATTTGTTCATCG 45.0 50.0 58.1 58.0 2787 bp 0.052632 40.8
clpP_p5 CTAGTACATGTTCCTCGGCG GCCGCCATATTTGTTCATCG 55.0 50.0 57.9 58.0 2841 bp 0.052632 40.8