Hotspot

ccsA-ndhD

SSC · 122476-122678 · 203 bp

Back to Rheum

Pi 0.0371
MarkerSeek score 65.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
203
variable_sites
22
pi
0.0371
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GGCCTATGCAGCCCTTTTAT GGTCCGCGAGAGTTATTTGT 50.0 50.0 58.0 58.0 850-886 bp 1.0 86.8
ccsA-ndhD_p2 GGCCTATGCAGCCCTTTTAT TCAACGGGGCCATTTTACAA 50.0 45.0 58.0 58.0 1239-1275 bp 1.0 85.3
ccsA-ndhD_p3 GGCCTATGCAGCCCTTTTAT TTCAACGGGGCCATTTTACA 50.0 45.0 58.0 58.0 1240-1276 bp 1.0 85.3
ccsA-ndhD_p4 GGCCTATGCAGCCCTTTTAT GGTTGATGATAGCAGGCACA 50.0 50.0 58.0 58.0 1386-1422 bp 1.0 84.8
ccsA-ndhD_p5 GGCCTATGCAGCCCTTTTAT TGGTTGATGATAGCAGGCAC 50.0 50.0 58.0 58.0 1387-1423 bp 1.0 84.8