Hotspot

rpl32-trnL-UAG

SSC · 120422-121293 · 872 bp

Back to Rheum

Pi 0.0197
MarkerSeek score 51.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
872
variable_sites
50
pi
0.0197
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.43
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCCGCATTAAAAGCTTTT GGCGCTGTTATTGCACTTAC 45.0 50.0 58.3 57.8 950-1497 bp 1.0 86.6
rpl32-trnL-UAG_p2 CGGCCGCATTAAAAGCTTTT GCCCCGAATAAATCCAACGA 45.0 50.0 58.3 58.3 832-1379 bp 1.0 85.6
rpl32-trnL-UAG_p3 CGGCCGCATTAAAAGCTTTT GGATTGGAGAGCGGGTACTA 45.0 55.0 58.3 58.3 1038-1585 bp 1.0 85.4
rpl32-trnL-UAG_p4 CGGCCGCATTAAAAGCTTTT TGGGTAAAAATAGCACCGGG 45.0 50.0 58.3 57.9 980-1515 bp 0.894737 82.3
rpl32-trnL-UAG_p5 CACCGGCTCTTACCTCTTTC CACGTTTCCTTTGGATCCCA 55.0 50.0 58.0 58.1 2535-2973 bp 0.736842 78.0