Hotspot

ndhF-rpl32

SSC · 119291-120247 · 957 bp

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Pi 0.0275
MarkerSeek score 77.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
957
variable_sites
114
pi
0.0275
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGCTCCGATTAACAAAGGA GCCGAATGTCCTTTTCTTTTCC 50.0 45.5 58.0 58.4 1067-1319 bp 1.0 72.2
ndhF-rpl32_p2 TCCCGCTCCGATTAACAAAG GCCGAATGTCCTTTTCTTTTCC 50.0 45.5 58.0 58.4 1069-1321 bp 1.0 72.2
ndhF-rpl32_p3 GTCCCGCTCCGATTAACAAA GCCGAATGTCCTTTTCTTTTCC 50.0 45.5 58.3 58.4 1070-1322 bp 1.0 70.2
ndhF-rpl32_p4 GAAGTCCCGCTCCGATTAAC GCCGAATGTCCTTTTCTTTTCC 55.0 45.5 58.4 58.4 1073-1325 bp 1.0 68.9
ndhF-rpl32_p5 CCGCTCCGATTAACAAAGGA CCGAATGTCCTTTTCTTTTCCA 50.0 40.9 58.0 57.2 1066-1318 bp 1.0 68.9