Hotspot

rpl33-rps18

LSC · 69364-69837 · 474 bp

Back to Rheum

Pi 0.0226
MarkerSeek score 59.40
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
474
variable_sites
29
pi
0.0226
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.96
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CAGCCGGTTGGAATTGAAAA CGCTTAGGTTTAAGTAAAGATCGC 45.0 41.7 57.2 58.3 499-585 bp 1.0 48.7
rpl33-rps18_p2 CAGCCGGTTGGAATTGAAAA CCGCTTAGGTTTAAGTAAAGATCG 45.0 41.7 57.2 57.7 500-586 bp 1.0 48.6
rpl33-rps18_p3 CAGCCGGTTGGAATTGAAAA GGTTTAAGTAAAGATCGCTTGGA 45.0 39.1 57.2 56.9 493-579 bp 0.947368 46.8
rpl33-rps18_p4 CAGCCGGTTGGAATTGAAAA AGGTTTAAGTAAAGATCGCTTGG 45.0 39.1 57.2 56.9 494-580 bp 0.947368 46.8
rpl33-rps18_p5 CAGCCGGTTGGAATTGAAAA AGGTTTAAGTAAAGATCGCTTGGA 45.0 37.5 57.2 58.2 494-580 bp 0.947368 46.3