Hotspot

atpB-rbcL

LSC · 55851-56642 · 792 bp

Pi 0.0139

MarkerSeek score 67.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 46
pi: 0.0139
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACACGCCCCAGGTTATTTTT	GCTTTGAATCCAACACCTGC	45.0	50.0	58.0	57.9	855-946 bp	1.0	87.5
atpB-rbcL_p2	CTTGCCTGGTGGAAAAGCTA	GCTTTGAATCCAACACCTGC	50.0	50.0	58.1	57.9	904-995 bp	1.0	86.7
atpB-rbcL_p3	CCAATGATTTGAGCGACACG	GCTTTGAATCCAACACCTGC	50.0	50.0	57.8	57.9	870-961 bp	1.0	86.2
atpB-rbcL_p4	GCCTGGTGGAAAAGCTACAT	GCTTTGAATCCAACACCTGC	50.0	50.0	58.2	57.9	901-992 bp	1.0	86.1
atpB-rbcL_p5	TTGCCTGGTGGAAAAGCTAC	GCTTTGAATCCAACACCTGC	50.0	50.0	58.4	57.9	903-994 bp	1.0	84.3