Hotspot

psbM-trnD-GUC

LSC · 30399-31333 · 935 bp

Back to Rheum

Pi 0.0147
MarkerSeek score 66.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
935
variable_sites
60
pi
0.0147
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 CGTTTTGGCTGACCGTTTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 58.1 58.5 999-1100 bp 1.0 84.1
psbM-trnD-GUC_p2 TAATCGTTTTGGCTGACCGT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.8 58.5 1003-1104 bp 1.0 83.3
psbM-trnD-GUC_p3 CGTTTTGGCTGACCGTTTTT GTTCAATTGGTCAGAGCACC 45.0 50.0 58.1 57.0 1000-1101 bp 1.0 79.9
psbM-trnD-GUC_p4 AATCGTTTTGGCTGACCGTT TTCAATTGGTCAGAGCACCG 45.0 50.0 58.7 58.5 1002-1103 bp 1.0 79.3
psbM-trnD-GUC_p5 ATCGTTTTGGCTGACCGTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 58.7 58.5 1001-1102 bp 1.0 79.3