Hotspot

petN-psbM

LSC · 29250-30293 · 1044 bp

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Pi 0.0148
MarkerSeek score 68.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1044
variable_sites
69
pi
0.0148
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CCCTTTCACTCGTCGTATGG AAAAACGGTCAGCCAAAACG 55.0 45.0 58.1 58.1 1107-1237 bp 1.0 87.3
petN-psbM_p2 CCTTTCACTCGTCGTATGGG AAAAACGGTCAGCCAAAACG 55.0 45.0 58.1 58.1 1106-1236 bp 1.0 87.3
petN-psbM_p3 CTTTCACTCGTCGTATGGGG AAAAACGGTCAGCCAAAACG 55.0 45.0 58.1 58.1 1105-1235 bp 1.0 87.3
petN-psbM_p4 GTATGGGGGAGAAGTGGACT AAAAACGGTCAGCCAAAACG 55.0 45.0 58.1 58.1 1093-1223 bp 1.0 87.0
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT AAAAACGGTCAGCCAAAACG 50.0 45.0 58.2 58.1 1152-1282 bp 1.0 86.5