Hotspot

trnR-UCU-atpA

LSC · 11010-11194 · 185 bp

Back to Rheum

Pi 0.0422
MarkerSeek score 75.90
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
185
variable_sites
35
pi
0.0422
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC CATTCACTGAGGAAGCGGAA 47.6 50.0 57.3 57.9 267-349 bp 1.0 77.7
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC AGACATTCACTGAGGAAGCG 47.6 50.0 57.3 57.6 270-352 bp 1.0 75.4
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC ATTCACTGAGGAAGCGGAAG 47.6 50.0 57.3 57.6 266-348 bp 1.0 75.3
trnR-UCU-atpA_p4 TGGATAGGACAGAGGTCTTCT CATTCACTGAGGAAGCGGAA 47.6 50.0 56.9 57.9 302-384 bp 1.0 74.8
trnR-UCU-atpA_p5 AGTCGTAATGATAAGCGTCCA CATTCACTGAGGAAGCGGAA 42.9 50.0 56.9 57.9 331-413 bp 1.0 74.7