Hotspot

trnH-GUG-psbA

LSC · 77-457 · 381 bp

Back to Rheum

Pi 0.0648
MarkerSeek score 83.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
381
variable_sites
112
pi
0.0648
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 355-590 bp 1.0 90.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 369-604 bp 1.0 90.7
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 368-603 bp 1.0 90.7
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 370-605 bp 1.0 90.7
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCCCCTAGACTTAGCTGCTA 55.0 55.0 58.1 57.6 306-541 bp 1.0 87.9