Hotspot

ycf1

SSC · 128654-134311 · 5658 bp

Back to Rhaphiolepis

Pi 0.00169

MarkerSeek score 44.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5658
variable_sites: 26
pi: 0.00169
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTCCTCATACCATTCCGGA	AAAGACAAAAGAAAGCGCGA	50.0	40.0	57.5	56.9	242 bp	1.0	68.2
ycf1_p2	AGTCCTCATACCATTCCGGA	AAGACAAAAGAAAGCGCGAA	50.0	40.0	57.5	56.9	241 bp	1.0	68.2
ycf1_p3	AGTCCTCATACCATTCCGGA	AGACAAAAGAAAGCGCGAAA	50.0	40.0	57.5	56.9	240 bp	1.0	68.2
ycf1_p4	AGTCCTCATACCATTCCGGA	AAGACAAAAGAAAGCGCGAAA	50.0	38.1	57.5	57.6	241 bp	1.0	65.9
ycf1_p5	AGTCCTCATACCATTCCGGA	AGACAAAAGAAAGCGCGAAAA	50.0	38.1	57.5	57.6	240 bp	1.0	65.9