Hotspot

rps16

LSC · 5184-6321 · 1138 bp

Back to Rhaphiolepis

Pi 0.00199

MarkerSeek score 51.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1138
variable_sites: 6
pi: 0.00199
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAAACAAAAAGGTTATACAAGGCT	ACTGGTTCCAAACTGAAGGA	32.0	45.0	57.4	56.6	1219-1223 bp	1.0	40.5
rps16_p2	ACAAAAAGGTTATACAAGGCT	ACTGGTTCCAAACTGAAGGA	33.3	45.0	53.3	56.6	1215-1219 bp	1.0	40.5
rps16_p3	GGAAATAGCAAAGAAACAAAAAGGT	ACTGGTTCCAAACTGAAGGA	32.0	45.0	57.2	56.6	1230-1234 bp	1.0	40.5
rps16_p4	AGCAAAGAAACAAAAAGGTT	ACTGGTTCCAAACTGAAGGA	30.0	45.0	52.2	56.6	1224-1228 bp	1.0	40.5
rps16_p5	AGAACAAAAGAGGAAATAGCAAAGA	ACTGGTTCCAAACTGAAGGA	32.0	45.0	57.2	56.6	1241-1245 bp	1.0	40.5