Hotspot

petB

LSC · 78225-79671 · 1447 bp

Back to Rhaphiolepis

Pi 0.00133

MarkerSeek score 37.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1447
variable_sites: 4
pi: 0.00133
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTGGTAGTTTGACCGTGGAA	AGTGTTCCTACCCCAAGTGA	45.0	50.0	57.0	57.9	1540-1566 bp	1.0	71.6
petB_p2	TTTGGTAGTTTGACCGTGGA	AGTGTTCCTACCCCAAGTGA	45.0	50.0	57.0	57.9	1541-1567 bp	1.0	71.6
petB_p3	TGGTAGTTTGACCGTGGAAT	AGTGTTCCTACCCCAAGTGA	45.0	50.0	56.8	57.9	1539-1565 bp	1.0	69.7
petB_p4	TTTGGTAGTTTGACCGTGGAA	AGTGTTCCTACCCCAAGTGA	42.9	50.0	57.7	57.9	1541-1567 bp	1.0	69.4
petB_p5	TTTTGGTAGTTTGACCGTGGA	AGTGTTCCTACCCCAAGTGA	42.9	50.0	57.7	57.9	1542-1568 bp	1.0	69.4