Hotspot

rpl22

LSC · 88981-89385 · 405 bp

Back to Rhamnus

Pi 0.00353

MarkerSeek score 32.50

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 4
pi: 0.00353
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCCATAATCCCCCGCTACTA	GGGCCCCAATTGGTAGAAAA	55.0	50.0	58.1	58.1	1023-1131 bp	1.0	84.5
rpl22_p2	CCCATAATCCCCCGCTACTA	CCGGATCGAAATGTTGGCTA	55.0	50.0	58.1	58.1	1113-1221 bp	1.0	84.1
rpl22_p3	CCCATAATCCCCCGCTACTA	GGTAGGCCATAAATTGGGGG	55.0	55.0	58.1	58.0	709-746 bp	1.0	81.8
rpl22_p4	CCCATAATCCCCCGCTACTA	TCCCGAGCGTCTACCATTAT	55.0	50.0	58.1	58.0	804-841 bp	1.0	81.7
rpl22_p5	CCCATAATCCCCCGCTACTA	AATTGGGGGAATTTGCACCT	55.0	45.0	58.1	58.0	698-735 bp	1.0	81.6