Hotspot

petA-psbJ

LSC · 67602-68671 · 1070 bp

Back to Rhamnus

Pi 0.00774
MarkerSeek score 52.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1070
variable_sites
23
pi
0.00774
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAGAAGGTTCAATTGTCCGA TCTTGTGATCGGTTTAATCGGT 42.9 40.9 57.2 57.8 1161-1186 bp 1.0 51.3
petA-psbJ_p2 TGAGAAGGTTCAATTGTCCGA ACTGGTATTCTTGTGATCGGT 42.9 42.9 57.2 56.7 1169-1194 bp 1.0 50.7
petA-psbJ_p3 TGAGAAGGTTCAATTGTCCGAA TCTTGTGATCGGTTTAATCGGT 40.9 40.9 57.9 57.8 1161-1186 bp 1.0 48.7
petA-psbJ_p4 TTGAGAAGGTTCAATTGTCCGA TCTTGTGATCGGTTTAATCGGT 40.9 40.9 57.9 57.8 1162-1187 bp 1.0 48.7
petA-psbJ_p5 TGAGAAGGTTCAATTGTCCGA ACTGGTATTCTTGTGATCGGTT 42.9 40.9 57.2 57.4 1169-1194 bp 1.0 48.4