Hotspot

psaI-ycf4

LSC · 64161-64632 · 472 bp

Back to Rhamnus

Pi 0.00375

MarkerSeek score 63.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 472
variable_sites: 5
pi: 0.00375
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	593-615 bp	1.0	82.6
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	596-618 bp	1.0	82.6
psaI-ycf4_p3	GGGCTTAGTATTTCCGGCAA	GGATAAAGGCCCAGCAGAAA	50.0	50.0	58.0	57.9	602-624 bp	1.0	82.4
psaI-ycf4_p4	GGGCTTAGTATTTCCGGCAA	AAAGGATAAAGGCCCAGCAG	50.0	50.0	58.0	57.9	605-627 bp	1.0	82.3
psaI-ycf4_p5	CGGCAATTGCAATGGTTTCT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	588-610 bp	1.0	82.2