Hotspot

rps4-trnT-UGU

LSC · 49802-50265 · 464 bp

Back to Rhamnus

Pi 0.00647

MarkerSeek score 64.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 9
pi: 0.00647
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	579-607 bp	1.0	82.0
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	583-611 bp	1.0	81.6
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	GGGGTTAGAGCATCGCATTT	50.0	50.0	58.0	58.3	607-635 bp	1.0	81.2
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	GGGGTTAGAGCATCGCATTT	50.0	50.0	58.1	58.3	611-639 bp	1.0	80.8
rps4-trnT-UGU_p5	GCTTCCGGCTCTAGGTTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.2	57.8	572-600 bp	1.0	80.8