Hotspot

trnT-GGU-psbD

LSC · 34558-35885 · 1328 bp

Back to Rhamnus

Pi 0.00368
MarkerSeek score 62.90
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1328
variable_sites
13
pi
0.00368
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CCGCAACCAGTCATCCATAA 55.0 50.0 58.1 58.0 1443-1475 bp 1.0 81.2
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CCGCAACCAGTCATCCATAA 50.0 50.0 58.1 58.0 1446-1478 bp 1.0 81.0
trnT-GGU-psbD_p3 AACGGGCCCTTTTAACTCAG CCGCAACCAGTCATCCATAA 50.0 50.0 58.1 58.0 1462-1494 bp 1.0 80.9
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1463-1495 bp 1.0 79.5
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA CCGCAACCAGTCATCCATAA 50.0 50.0 58.3 58.0 1420-1452 bp 1.0 79.4