Hotspot

trnS-GCU-trnG-UCC

LSC · 8948-10226 · 1279 bp

Back to Rhamnus

Pi 0.00877
MarkerSeek score 63.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1279
variable_sites
31
pi
0.00877
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TTAATCAAACCGAGGGACCC 50.0 50.0 58.0 57.2 1322-1419 bp 1.0 79.4
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TTAATCAAACCGAGGGACCC 50.0 50.0 58.0 57.2 1321-1418 bp 1.0 79.4
trnS-GCU-trnG-UCC_p3 CAAAGTGTCGACGAAAACGG TTAATCAAACCGAGGGACCC 50.0 50.0 58.0 57.2 1392-1489 bp 1.0 79.3
trnS-GCU-trnG-UCC_p4 AAGTGTCGACGAAAACGGAA TTAATCAAACCGAGGGACCC 45.0 50.0 58.1 57.2 1390-1487 bp 1.0 78.8
trnS-GCU-trnG-UCC_p5 AGTGTCGACGAAAACGGAAA TTAATCAAACCGAGGGACCC 45.0 50.0 58.1 57.2 1389-1486 bp 1.0 78.8