Hotspot

rps16-trnQ-UUG

LSC · 7065-7778 · 714 bp

Back to Rhamnus

Pi 0.0024

MarkerSeek score 60.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 6
pi: 0.0024
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GTCGAATAGATTTGGGGCGT	50.0	50.0	57.9	58.1	863-877 bp	1.0	80.1
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	TCGAATAGATTTGGGGCGTG	50.0	50.0	57.9	58.1	862-876 bp	1.0	80.1
rps16-trnQ-UUG_p3	AACGGATCGTGTCCTTCAAG	TGTCGAATAGATTTGGGGCG	50.0	50.0	57.9	58.1	864-878 bp	1.0	80.1
rps16-trnQ-UUG_p4	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	803-817 bp	1.0	80.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTCGAATAGATTTGGGGCGT	45.0	50.0	58.1	58.1	846-860 bp	1.0	79.7