Hotspot

trnK-UUU-rps16

LSC · 4892-5900 · 1009 bp

Back to Rhamnus

Pi 0.00856

MarkerSeek score 67.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1009
variable_sites: 23
pi: 0.00856
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	57.9	58.0	1189-1204 bp	1.0	83.4
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	57.9	58.0	1188-1203 bp	1.0	83.4
trnK-UUU-rps16_p3	CCGATGGTATGGACGAATCC	ATTTCAAAGAAAGCGGGGGT	55.0	45.0	57.9	58.0	1167-1182 bp	1.0	83.3
trnK-UUU-rps16_p4	AGTCGCGGTCTTACAAACTC	TCCTGCAAAAAGGAGCTCAG	50.0	50.0	57.9	58.1	1230-1245 bp	1.0	82.5
trnK-UUU-rps16_p5	GTCGCGGTCTTACAAACTCT	TCCTGCAAAAAGGAGCTCAG	50.0	50.0	57.9	58.1	1229-1244 bp	1.0	82.5