Hotspot

rpl32-trnL-UAG

SSC · 118853-120355 · 1503 bp

Back to Rhamnella

Pi 0.0451

MarkerSeek score 76.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1503
variable_sites: 112
pi: 0.0451
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGAAGTCTCTTTCTACGAGG	AGCGTGTCTACCGATTTCAC	52.4	50.0	58.2	58.0	1257-1618 bp	1.0	80.4
rpl32-trnL-UAG_p2	GCGAAGTCTCTTTCTACGAGG	CAGCGTGTCTACCGATTTCA	52.4	50.0	58.2	58.0	1258-1619 bp	1.0	80.4
rpl32-trnL-UAG_p3	GCGAAGTCTCTTTCTACGAGG	TCCTAAGAGCAGCGTGTCTA	52.4	50.0	58.2	57.9	1267-1628 bp	1.0	79.4
rpl32-trnL-UAG_p4	GCGAAGTCTCTTTCTACGAGG	TGCCTTTTTCGGACGAATCA	52.4	45.0	58.2	58.1	1565-1926 bp	1.0	78.9
rpl32-trnL-UAG_p5	GCGAAGTCTCTTTCTACGAGG	GCCGCATAGCTTAAGACCAT	52.4	50.0	58.2	58.1	1890-2245 bp	1.0	78.3