Hotspot

ndhC-trnV-UAC

LSC · 54621-55154 · 534 bp

Back to Rhamnella

Pi 0.0412

MarkerSeek score 75.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 38
pi: 0.0412
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGTAGCAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.3	58.7	575-599 bp	1.0	49.2
ndhC-trnV-UAC_p2	TCATATTCGTAAAGTAGCAACA	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.3	57.8	587-611 bp	1.0	49.1
ndhC-trnV-UAC_p3	TCATATTCGTAAAGTAGCAACA	GAAGGTCTACGGTTCGAGTC	31.8	55.0	53.3	57.5	586-610 bp	1.0	49.1
ndhC-trnV-UAC_p4	ATCATATTCGTAAAGTAGCAACA	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.0	57.8	588-612 bp	1.0	49.1
ndhC-trnV-UAC_p5	TCATATTCGTAAAGTAGCAACAT	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.0	57.8	587-611 bp	1.0	49.1