Hotspot

psbZ-trnG-GCC

LSC · 38656-39205 · 550 bp

Back to Rhamnella

Pi 0.0431

MarkerSeek score 81.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 550
variable_sites: 45
pi: 0.0431
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	712-796 bp	1.0	84.8
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	713-797 bp	1.0	84.8
psbZ-trnG-GCC_p3	CTGATGGTTGGGCGGATAAT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	703-787 bp	1.0	84.3
psbZ-trnG-GCC_p4	CTGATGGTTGGGCGGATAAT	GAATCGAACCCGCATCTTCT	50.0	50.0	57.7	58.1	704-788 bp	1.0	84.3
psbZ-trnG-GCC_p5	CCGTCGTATTTGCTTCTCCT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	721-805 bp	1.0	84.0