Hotspot

trnT-GGU-psbD

LSC · 34182-35369 · 1188 bp

Back to Rhamnella

Pi 0.032

MarkerSeek score 78.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 66
pi: 0.032
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCGCAACCAGTCATCCATAA	55.0	50.0	58.1	58.0	1318-1467 bp	1.0	86.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1321-1470 bp	1.0	86.6
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1337-1486 bp	1.0	86.5
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.3	58.0	1295-1444 bp	1.0	85.2
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1338-1487 bp	1.0	85.0