Hotspot

psbK-psbI

LSC · 7892-8350 · 459 bp

Back to Rhamnella

Pi 0.0344

MarkerSeek score 74.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 29
pi: 0.0344
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	CCCCGGATCGTTAGATAGGA	50.0	55.0	57.9	57.8	566-577 bp	1.0	85.3
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	CCCCGGATCGTTAGATAGGA	45.0	55.0	58.1	57.8	571-582 bp	1.0	85.2
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	CCCCGGATCGTTAGATAGGA	45.0	55.0	58.3	57.8	563-574 bp	1.0	83.3
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	CCCCGGATCGTTAGATAGGA	45.0	55.0	58.3	57.8	564-575 bp	1.0	83.3
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	CCCCGGATCGTTAGATAGGA	45.0	55.0	58.3	57.8	565-576 bp	1.0	83.2