Hotspot

rps16-trnQ-UUG

LSC · 6495-7265 · 771 bp

Back to Rhamnella

Pi 0.0452

MarkerSeek score 82.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 58
pi: 0.0452
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	808-844 bp	1.0	87.9
rps16-trnQ-UUG_p2	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	822-858 bp	1.0	86.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	812-848 bp	1.0	83.1
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	838-874 bp	1.0	82.9
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	800-836 bp	1.0	82.8