Hotspot

ycf1

SSC · 123452-128800 · 5349 bp

Back to Rhammatophyllum

Pi 0.0165

MarkerSeek score 54.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5349
variable_sites: 132
pi: 0.0165
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCCGCCGATTCTTTTTCTT	TTAGGGGTATGGGGGACATC	45.0	55.0	58.1	57.9	464 bp	1.0	81.8
ycf1_p2	ACCCGCCGATTCTTTTTCTT	ATTTACCTCAGGTTGCGTGG	45.0	50.0	58.1	58.2	779 bp	1.0	80.7
ycf1_p3	ACCCGCCGATTCTTTTTCTT	GGGACATCCGGCATAGAAAA	45.0	50.0	58.1	57.7	452 bp	1.0	80.1
ycf1_p4	ACCCGCCGATTCTTTTTCTT	GTTGCGTGGGCTAGATTAGT	45.0	50.0	58.1	57.7	768 bp	1.0	79.7
ycf1_p5	ACCCGCCGATTCTTTTTCTT	GGTTGCGTGGGCTAGATTAG	45.0	55.0	58.1	58.4	769 bp	1.0	78.8