Hotspot

ndhE-ndhG

SSC · 117701-117967 · 267 bp

Back to Rhammatophyllum

Pi 0.0433

MarkerSeek score 72.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 267
variable_sites: 16
pi: 0.0433
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCGACTCGTGATCAATCCA	AATTTCGGTGGCTCGTCAAT	45.0	45.0	56.7	58.2	358-365 bp	1.0	73.8
ndhE-ndhG_p2	TCGACTCGTGATCAATCCAT	AATTTCGGTGGCTCGTCAAT	45.0	45.0	56.5	58.2	357-364 bp	1.0	72.0
ndhE-ndhG_p3	TTTCGACTCGTGATCAATCCA	AATTTCGGTGGCTCGTCAAT	42.9	45.0	57.4	58.2	359-366 bp	1.0	71.6
ndhE-ndhG_p4	TTCGACTCGTGATCAATCCA	TTTCGGTGGCTCGTCAATAA	45.0	45.0	56.7	57.5	356-363 bp	1.0	71.6
ndhE-ndhG_p5	TTCGACTCGTGATCAATCCAT	AATTTCGGTGGCTCGTCAAT	42.9	45.0	57.2	58.2	358-365 bp	1.0	69.9